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ms2 p65 hsf1 gfp vector  (Addgene inc)


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    Addgene inc ms2 p65 hsf1 gfp vector
    Ms2 P65 Hsf1 Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+gfp+vector/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/bio_rxiv__64898__2026__03__01__708923-177-6-13
    Average 94 stars, based on 82 article reviews
    ms2 p65 hsf1 gfp vector - by Bioz Stars, 2026-10
    94/100 stars

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    Amplification:

    Article Title: Single-cell CRISPR activation screens in primary B cells discover gene regulatory mechanisms for hundreds of autoimmune risk loci
    Article Snippet: The subsequent dCas9-VP64-T2A-ChFP fragment was amplified and cloned into retroviral vector MSCV-BCL6-t2A-BCL2 (Addgene #135305; ( , )) digested with XhoI (NEB) and SalI-HF (NEB) to replace the BCL6-T2A-BCL2-IRES-CD2 fragment and generate MSCV-dCas9-VP64-ChFP . .. Similarly, MCP-p65-HSF1-T2A-GFP was amplified from the MS2-P65-HSF1_GFP vector (a gift from Feng Zhang; Addgene #61423 ( )) and cloned into MSCV-BCL6-t2A-BCL2 backbone to generate MSCV-MCP-p65-HSF1-GFP . .. ChFP in the lentiviral CROP-sgRNA-MS2 vector (a gift from Wolf Reik; Addgene #153457) was replaced with BFP by GenScript using their gene synthesis service to generate CROP-sgRNA-MS2-BFP .

    Clone Assay:

    Article Title: Single-cell CRISPR activation screens in primary B cells discover gene regulatory mechanisms for hundreds of autoimmune risk loci
    Article Snippet: The subsequent dCas9-VP64-T2A-ChFP fragment was amplified and cloned into retroviral vector MSCV-BCL6-t2A-BCL2 (Addgene #135305; ( , )) digested with XhoI (NEB) and SalI-HF (NEB) to replace the BCL6-T2A-BCL2-IRES-CD2 fragment and generate MSCV-dCas9-VP64-ChFP . .. Similarly, MCP-p65-HSF1-T2A-GFP was amplified from the MS2-P65-HSF1_GFP vector (a gift from Feng Zhang; Addgene #61423 ( )) and cloned into MSCV-BCL6-t2A-BCL2 backbone to generate MSCV-MCP-p65-HSF1-GFP . .. ChFP in the lentiviral CROP-sgRNA-MS2 vector (a gift from Wolf Reik; Addgene #153457) was replaced with BFP by GenScript using their gene synthesis service to generate CROP-sgRNA-MS2-BFP .

    Construct:

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data . ..

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system.
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data 1. ..

    Plasmid Preparation:

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data . ..

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system.
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data 1. ..

    Over Expression:

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data . ..

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system.
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data 1. ..

    Cloning:

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data . ..

    Article Title: Biased genome editing using the local accumulation of DSB repair molecules system.
    Article Snippet: .. For the effector vectors with MS2 coat protein, we first constructed MS2-Exo1 vector by the addition of MS2-NLS obtained from the MS2-P65-HSF1_GFP vector (Plasmid #61423, Addgene) to the Exo1 overexpression vector by the In-Fusion cloning method using the primers listed in Supplementary Data 1. ..



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    ms2 p65 hsf1 gfp vector  (Addgene inc)
    94
    Addgene inc ms2 p65 hsf1 gfp vector
    Ms2 P65 Hsf1 Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+gfp+vector/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/bio_rxiv__64898__2026__03__01__708923-177-6-13
    Average 94 stars, based on 1 article reviews
    ms2 p65 hsf1 gfp vector - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    ms2 expressing vector  (Addgene inc)
    94
    Addgene inc ms2 expressing vector
    A) Scheme of synergistic HDR Enhacer techonology: Cas9 (in blue) is fused to PRDM9 (green). Cas9 is associated with different HDR enhacers via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of HDR enhancers and the MS2 coat protein. B) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9, Cas9-PRDM9, Cas9 co-deliver with PRDM9 and Cas9-PRDM9 together with a HDR enhancing factors alone or in combination. C) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9-PRDM9 and Cas9-PRDM9 together with the best HDR enhancing factors. D) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9 or Cas9-PRDM9 together with MS2-CtlP. E) SHE performance for HDR in human iPS cells for HDR and NHEJ comparison, F) and relative measurements.
    Ms2 Expressing Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+p65+hsf1+gfp+vector/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/bio_rxiv__2022__12__05__519167-97-1-4
    Average 94 stars, based on 1 article reviews
    ms2 expressing vector - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    A) Scheme of synergistic HDR Enhacer techonology: Cas9 (in blue) is fused to PRDM9 (green). Cas9 is associated with different HDR enhacers via sgRNA loops that include MS2 phage aptamer sequences bound by fusion proteins of HDR enhancers and the MS2 coat protein. B) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9, Cas9-PRDM9, Cas9 co-deliver with PRDM9 and Cas9-PRDM9 together with a HDR enhancing factors alone or in combination. C) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9-PRDM9 and Cas9-PRDM9 together with the best HDR enhancing factors. D) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9 or Cas9-PRDM9 together with MS2-CtlP. E) SHE performance for HDR in human iPS cells for HDR and NHEJ comparison, F) and relative measurements.

    Journal: bioRxiv

    Article Title: Synergic homology directed recombination by PRDM9 meiotic factor

    doi: 10.1101/2022.12.05.519167

    Figure Lengend Snippet: A) Scheme of synergistic HDR Enhacer techonology: Cas9 (in blue) is fused to PRDM9 (green). Cas9 is associated with different HDR enhacers via sgRNA loops that include MS2 phage aptamer sequences bound by fusion proteins of HDR enhancers and the MS2 coat protein. B) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9, Cas9-PRDM9, Cas9 co-deliver with PRDM9 and Cas9-PRDM9 together with a HDR enhancing factors alone or in combination. C) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9-PRDM9 and Cas9-PRDM9 together with the best HDR enhancing factors. D) HDR/NHEJ ratio of the Traffic Light Reporter, upon transfection with Cas9 or Cas9-PRDM9 together with MS2-CtlP. E) SHE performance for HDR in human iPS cells for HDR and NHEJ comparison, F) and relative measurements.

    Article Snippet: Similarly, MS2 expressing vector (Addgene #61423) was also amplified by PCR.

    Techniques: Transfection, Comparison